Determination of Plasma Concentration of Paclitaxel in Tumor Patients by RP-HPLC / 中国药房
China Pharmacy
; (12)2005.
Article
em Zh
| WPRIM
| ID: wpr-532318
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WPRO
ABSTRACT
OBJECTIVE:To establish a RP-HPLC method to determinate the plasma concentration of paclitaxel in tumor patients.METHODS:Paclitaxel was extracted from plasma with organic phase(ethyl-acetate)by two-step extraction on Tianhe Kromasil C18 column(250 mm?4.6 mm,5 ?m)with a mobile phase consisted of acetonitrile-methanol-water(40∶25∶40)at a flow rate of 1 mL?min-1.The detective wavelength was set at 227 nm and the column temperature was maintained at 35 ℃.RESULTS:The linear range of paclitaxel was 0.05~5.00 mg?L-1(r=0.999 7)with average recovery rate at 98.75%~100.44%.Both intra-day RSD and inter-day RSD were less than 5%(n=5).The plasma concentration-time profile in 11 patients after iv infusion of paclitaxel was in line with a two-compartment model.CONCLUSION:This established method is simple,accurate,reproducible and applicable for clinical determination of blood drug concentration and pharmacokinetic studies.
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WPRIM
Idioma:
Zh
Revista:
China Pharmacy
Ano de publicação:
2005
Tipo de documento:
Article