Determination of Entrapment Efficiency of Gemcitabine Hydrochloride Liposome / 中国药房
China Pharmacy
;
(12)2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-532487
ABSTRACT
OBJECTIVE:
To establish a method for determining the entrapment efficiency of gemcitabine hydrochloride liposomes(GHL).METHODS:
The liposome and the free gemcitabine hydrochloride were separated by ultrafiltration,and the content of free gemcitabine hydrochloride was determined by HPLC.The entrapment efficiency of gemcitabine hydrochloride liposomes was computed as well.RESULTS:
The recovery rate in ultrafiltration methods was 97.8%~100.1% for blank substance versus 99.0%~100.1% for sample.The linear range of gemcitabine hydrochloride was 1.0~80.0 mg?L-1(r=0.999 3) and its average recovery rate was 98.7~101.2%,and its inter-day and intra-day RSD were all less than 3% and its average entrapment efficiency was 81.21%.CONCLUSION:
The method is simple,accurate and applicable for the determination of the entrapment efficiency of gemcitabine hydrochloride liposomes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS