Separation,Purification and Detection of Product of Bovine Hemoglobin Modified by Polyethylene Glycol / 中国药房
China Pharmacy
;
(12)2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-532507
ABSTRACT
OBJECTIVE:
Researching the appropriate methods to separation,purification and detection the mixture of PEG modifier SC-mPEG with BHB.METHODS:
Adopting cation-exchange chromatography and organic solvent precipitation to separate the mixture,then detecting it by SDS-PAGE and TNBS methods.RESULTS:
Modifer and non-modifer product were separation by cation-exchange chromatography.The average modification rates of two peak product from cation-exchange chromatography are 52.95% and 14.19% detected by TNBS methods.CONCLUSIONS:
Cation-exchange chromatography methods could be to separate and purify the SC-mPEG-BHB,TNBS methods coule be to detect the average modification rate of the SC-mPEG-BHB.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2005
Tipo de documento:
Artigo
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