Effect of cyclooxygenase-2 on hepatic injury and metabolism in rats with sepsis / 中国普通外科杂志

ABSTRACT

Objective To study the expression of cyclooxygenase2 in hepatic inflammatory reaction of rats with sepsis and its effect on metabolism.Methods Fifty-four Wistar rats were randomly divided into 3 groupsSham(A)group,sepsis model(B)group,and NS398 intervention(C)group.The rats were subjected to cecal ligation and puncture(CLP)for sepsis model or sham operation.RT-PCR was used to determine COX-2 mRNA expression,serum prealbumin(PA)and transferrin(TF)by radioimmunity.At the same time,ALT,AST,albumin determination and liver pathological changes were determined.Results(1)The expression of COX-2 mRNA was low in A group,higher in B and C groups,and higher in B than C group at each time period(P