Protective Effects of Shenqi Fuzheng Injection on Ischemia Reperfusion Injury of the Lung in Dogs Following Cardiopulmonary Bypass / 中国药房

ABSTRACT

OBJECTIVE:To study the protective effects of Shenqi fuzheng injection on ischemia/reperfusion injury of the lungs of dogs following cardiopulmonary bypass(CPB).METHODS:The CPB dog model was induced,then the model dogs we-re randomly divided into three groups:control group,Shenqi fuzheng injection high dosage group,and Shenqi fuzheng injection low dosage group. The lung tissue samples of 1.0 cm3 from the same lobes of lung were obtained at 10 min before CPB and 60 min and 120 min after reperfusion,respectively for determination of the contents of superoxide dismutase(SOD),malondialdehyde (MDA),wet / dry ratio of lung (W/D) etc. The femoral arterial blood gas analysis was performed in two groups at 30 min and 120 min after reperfusion for calculation of oxygenation index(OI) and respiratory index(RI).The lung tissue samples(1.0 cm3) from the same lobes of lung were obtained at 120 min after reperfusion for HE staining and observation of pathological changes under light microscope. RESULTS:In the control group,OI were significantly decreased but RI were sig-nificantly increased at 120 min after reperfusion as compared with at 30 min after reperfusion; in Shenqi fuzheng injection high dosage group at 30 min and 120 min,OI was significantly higher but RI was significantly lower than in control group. In the control group at 60,120 min after reperfusion,the SOD content were decreased significantly,MDA content and W/D ratio in lung tissues were increased significantly compared with at 10 min before CPB; in Shenqi fuzheng injection high dosage group at 60 min and 120 min,MDA content and W/D ratio were significantly lower but SOD content was significantly higher than in the control group.The pathological changes at 120 min after reperfusion showed that there were severe leukocyte infiltration and pulmonary edema in the control group,but the pathological changes in the Shenqi fuzheng injection high dosage group were milder than in the control group. CONCLUSION:Administration of Shenqi fuzheng injection in dog during CPB has beneficial protective effects against I/R lung injury,and the effect may be associated to its function in alleviating the damage induced by oxygen free radicals in lung tissues.