Simultaneous Determination of Isoniazid and Isonicotinic Acid in Isoniazid Tablets by HPLC / 中国药房
China Pharmacy
;
(12)1991.
Artigo
em Chinês
| WPRIM
| ID: wpr-533304
ABSTRACT
OBJECTIVE:
To establish an HPLC method for content determination of isoniazid and isonicotinic acid in isoniazid tablets.METHODS:
The determination was performed on SHIM-PACK VP-ODS C18 column.The mobile phase consisted of methanol-water(0.2% docusate sodium,4060) at a flow rate of 0.8 mL?min-1.The UV detection wavelength was set at 261 nm and the sample size was 5 ?L.RESULTS:
The linear range was 10~1000 ?g?mL-1 for isoniazid(r=0.999 8) with average recovery of 99.57%(RSD=0.26%) and 2~24 ?g?mL-1 for isonicotinic acid(r=0.9994) with average recovery of 98.96%(RSD=0.96%).The limited quantities were 0.05 ?g?mL-1,0.16 ?g?mL-1,respectively.CONCLUSION:
The established method is simple,rapid and accurate,and suitable for content determination of isoniazid tablets.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
1991
Tipo de documento:
Artigo
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