Expression of T cell-specific transcriptional factor Elf-1 in mononu-clear cells,CD4~+ T cells and CD8~+ T cells from umbilical cord blood / 中国病理生理杂志

ABSTRACT

AIM:To investigate the expression level of transcriptional factor Elf-1 in mononuclear cells,CD4+ and CD8+ T cells from umbilical cord blood (UCB). METHODS: Real-time PCR with SYBR green I technique was used for detecting the Elf-1 expression in mononuclear cells,sorted CD4+ and CD8+ T cells from 12 cases of umbilical cord blood. The relative mRNA expression level of Elf-1 was analyzed by a formula of 2-△Ct?100%. The expression level of ?2-microglobulin gene (?2M) was used as an endogenous reference. The peripheral blood from 10 cases of healthy adults was served as control. RESULTS: Elf-1 mRNA expressed in all blood samples collected from both UCB and healthy adults. The expression level showed apparent diversity in different individuals. The relative mRNA expression of Elf-1 in both mononuclear cells (18.55%?2.48%) and CD8+T cells (3.52%?0.45%) from UCB were significantly higher than those from healthy adults (9.16%?1.92%,2.02%?0.27%,respectively,P