Role of prostaglandin E_2 receptor 1 in neuronal cell death induced by hypoxia on rat cortical neurocytes / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Artigo
em Chinês
| WPRIM
| ID: wpr-534019
ABSTRACT
AIM:
To observe the effects of prostaglandin E2 receptor1 (EP1) in neuronal cell death induced by hypoxia/reoxygenation and ischemia/reperfusion.METHODS:
The cortical neurocytes of neonatal Wistar rats were cultured for 12 days and exposed to hypoxia/re-oxygenation to establish a hypoxia/re-oxygenation model. Another set of cultured primary neonatal cortical neurocytes of rats were pretreated with 17-pt (an antagonist of EP1),then underwent hypoxia for 3 h,re-oxygenated for 21 h. MTT reagent was added 1 h before measuring the cell viability. Neuron apoptosis was determined by flow cytometry. The protein expression was examined by Western blotting.RESULTS:
Compared to the control cells (only underwent hypoxia /re -oxygenation and without any pretreatment),the neurons pretreated with 17-pt and then underwent hypoxia/re-oxygenation showed significantly lower survival rate (P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1989
Tipo de documento:
Artigo
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