Determination of Psoralen and Isopsoralen in Laonian Kechuan Tablets by RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for content determination of psoralen and isopsoralen in Laonian kechuan tablets.METHODS:RP-HPLC was applied.The separation was performed on Kromasil C18(250 mm?4.6 mm,5 ?m) column with mobile phase of methanol-water(5545) at flow rate of 1.0 mL?min-1.The detection wavelength was set at 246 nm.RESULTS:The linear range of psoralen were 0.047 0～0.940 0 ?g with an average recovery of 97.70%(RSD=0.89%,n=9).The linear range of isopsoralen were 0.046 0～0.920 0 ?g with an average recovery of 98.76%(RSD=0.84%,n=9).CONCLUSION:The method is simple,rapid and accurate for the quality control of Laonian kechuan tablets.