Studies on the Heterogeneity of Antigen-Presenting Cells. Establishment of T cell Hybridomas specific for Different Antigen-Presenting Cells / 中国免疫学杂志

ABSTRACT

A series of alloreactive T hybridomas were obtained using fusion between BW147 and C3H/HeN T cell lymphoblasts, which were prepared by stimulating With lpsactivatedBalb/c B cells Six of the total 89 hybridomas tested have been shown to respond selectively to LPS-activated B cells, but not spleen adherent cells, macrophage tumor line and Ia-transfected fibroblasts. The results indicated: 1) Different Antigen-Presenting Cells could selectively stimulate the allogeneic responses of distinctive clonal T cells; 2) The specific stimulating effects may either due to the structural differences among the Ir-genes of different cell types, or the same gene produce(s), which may form different molecular complexes on the membrane surfaces of different cell types, lead to their functional specificities.