SEQUENTIAL DETERMINATION OF SPECIFIC IGM ANTIBODIES IN SERUM SAMPLES FROM PATIENTS WITH EPIDEMIC HEMORRHAGIC FEVER / 西安交通大学学报(医学版)

ABSTRACT

The specific IgM (SIgM) antibodies in serum samples from 32 patients with epidemic hemorrhagic fever (EHF) were sequentially determined by IgM antibody capture ELISA (MacELISA) and indirect immunofluorescence technique (IFA) sitnultaneously. Of the samples detected by the two methods, 99.46% had the same positive or negative results. The response curves of SIgM titres separately determined by MacELISA or IFA were parellel, whereas, the SIgM titres detected by MacELISA were comparatively higher than those detected by IFA. During the course of the disease, no significant defference was found between the SIgM titres of defferent illness types at the same illness day.