A RADIORECEPTOR ASSAY FOR HUMAN TSH RECEPTOR ANTIBODIES AND ITS CLINICAL APPLICATIONS / 中华内分泌代谢杂志

ABSTRACT

A radioreceptor assay for TRAb was described in which soluble TSH receptors obtained by solubilizing porcine thyroid membranes using 0.5% Triton X-100 and 125I-bTSH prepared by iodogen method and receptor-purification were used. The binding of 125I-bTSH to TSH receptors was inhibited by unlabeled bTSH and sera of patients with uncon-trolled Graves' disease (GD) in a dose-dependent manner. The assay was sensitive, specific, rapid and reproducible with interassay CV of 12.6% and 3.1% at mean inhibition values of 15.4% and 90.6% .respectively, being suitable for a clioical routine test. Of 133 patients with uncontrolled GD, TRAb was positive in 94.7%, while the absence of detectable TRAb was found in 6 patients with hyperthyroidism but not due to GD. This indicated TRAb measurement has important value in etiology and diagnosis of hyperthyroidism duc to GD.