Morphological observation on allografts of ieydig cells in castrated rats / 中华器官移植杂志

ABSTRACT

Leydig cells derived from a SD rat aged one month were transplanted to the hind leg subcutaneous of a 2 week-castrated outbred rat. At 1, 3, 4, 6 months after implantation, the grafts were removed and taken to have a histochemical staining for 3?-hydroxygsteroid dehydrogenase (3b-HSD), light and electron microscopy. The results indicated that there were a lots of 3b-HSD positive leying cells in the grafts. The well revascularized and no lymphocytic infiltration were seen around the implanted cells with 4 months after transplantation. Electron microscopy displayed that the implanted cells had abundant mitochondria and smooth endoplasm reticulum, suggesting that the implanted ceils could survive and had better secretary testosterone function for 4 months.