Effects of 4 sorts of growth factors on the cell growth of androgen independent prostate cancer cell line PC-3M / 中华老年医学杂志

ABSTRACT

Objective To evaluate the effects of TGF ? 1, EGF, bFGF, KGF on the cell growth of androgen independent prostate cancer cell line PC 3M in vitro. Methods We cultured androgen independent prostate cancer cell line PC 3M in vitro and added TGF ? 1, EGF, bFGF, KGF to the medium at different concentrations of 1, 5,10 and 50 ng/ml. Brdu incorporation method was used to determine the status of cell proliferation. Results The absorbance ( A ) value of the control group PC 3M was 0 759. Seventy two hours after adding the growth factors separately, the inhibition was enhanced with the increasing of concentrations. At the dosage of 50 ng/ml, A value decreased to 0 400(TGF ? 1),0 455(EGF),0 532(bFGF),and 0 491(KGF),respectively, showing significant difference compared to the controls. When the mixture of 1 ng/ml EGF with 0 5 ng/ml or 5 ng/ml TGF ? 1 was added into the medium, A values were 0 522 and 0 299, respectively. Conclusions All these four growth factors inhibited the proliferation of PC 3M cell line in a dose dependent manner. TGF ? 1 and EGF have no interaction to the cell proliferation.