Study on Endocrine Disruptor Chemicals Causing Malformed Frogs / 环境与健康杂志

ABSTRACT

Objective To study endocrine disruptor chemicals causing malformed frogs in an irrigation canal in T city and a pool in CH city. Methods Water samples collected from an irrigation canal in T city and a pool in CH city where malformed frogs were found were concentrated through H103 resin. The concentrated samples were purified and derivated. The endocrine disruptor chemicals in the derivatives were determined by GC/MS and the estrogenic activities in concentrated water samples were assessed by the recombinant yeast estrogen system (RYES). Results Endocrine disruptor chemicals bisphenol_A (5.6?10-3～1.52 ?g/L) and nonylphenol (0.46～0.58 ?g/L) were found in the concentrated water samples of the irrigation canal in T city and the pool in CH city. RYES showed that the estrogenic activities were positive and this result accorded with the data given by GC/MS. Conclusion The results indicated that the cause resulted in malformation of frogs in the irrigation canal in T city and the pool in CH city might be related to endocrine disruptor chemicals.