The role of FASL and TRAIL in the AICD of PBLs in patients with chronic hepatitis B / 中国免疫学杂志

ABSTRACT

Objective:To investigate the role of FASL and TRAIL in the AICD (activation induced cell death) of PBLs in patients with chronic hepatitis B.Methods:The PBLs of 20 nonnal control,24 patients with chronic hepatitis B and 24 patients with chronic severe hepatitis B were isolated and cultured with or without phytohemagglutinin( 10 pig/ml) for 48 hours in vitro. After incubation,the cells were harvested by centrifugation and the expression of FASL.,TRAIL in PBLs was assayed by reverse transcriptase-polymerase chain reaction (RT-PCR) and im-munohistochemical staining (SABC Method) .Results-.The expression of FASL mRNANTRAIL mRNA was undetectable in the resting PBLs of three investigated groups, but it was obviously increased after PHA stimulation in vitro. In comparison with the group of normal controls, the expression of FASL mRNA,TRAIL mRNA in PBLs was significantly higher in the group of patients with chronic hepatitis B( P