Effect of human cementum extract prepared by guanidine on the attachment of gingival fibroblasts and osteoblasts / 实用口腔医学杂志

ABSTRACT

Objective: To study the bioactivity of human cementum extract . Methods: Cementum was harvested from freshly extracted heatlhy teeth and cementum extract was prepared by guanidine. Human gingival fibroblast and MC3T3 E1 osteoblast were cultured. The cells were incubated in DMEM with cementum extract concentration 0, 2.5, 5, 10 or 20 ?g/ml for an hour respectively, the cell attachment rate was measured by cell counting. The attachment rate of the cells in different incubation time(30 min,60 min, 90 min, 120 min) in DMEM containing 10 ?g/ml cementum extract was also assayed.Results: The extract increased the cell attachment rate in a concentration and time dependent effect. The extract at the concentration of 10 ?g/ml and with exposuse time of 90 min gave the most effective increase of cell attachment. Conclusion: Human cementum extract prepared by guanidine contains some bioactive proteins that promote the attachment of gingival fibroblasts and osteoblasts.