Adenosine triphosphatase mechanism of calcium overload in vascular smooth muscle cell in hemorrhagic shock rats / 中华创伤杂志

ABSTRACT

Objective To explore the mechanism of adenosine triphosphatase (ATPase) of calcium overload in vascular smooth muscle cell (VSMC) in hemorrhagic shock rats. Methods Twenty-four male Wistar rats were randomly divide into four groups control (Group C), shock start (Group S0), 2 hours post-shock (Group S2) and 4 hours post-shock (Group S4). The rat models with hemorrhage shock were produced by means of modified Wigger's method through bloodletting from femoral artery and keeping blood pressure at 40 mm Hg for 2 hours. The changes of cytosolic free Ca 2+ concentration (i) and the activities of Ca 2+ - ATPase and Na +-K +- ATPase in VSMC membrane and mitochondrial membrane were monitored in rats after shock. Results Mean channel fluorescence values of VSMC i in the Groups C, SO, S2 and S4 were 2.03?0.15, 2.37? 0.32 , 2.55?0.46 and 2.80?0.43 respectively and increased in a time-dependent fashion. Mean channel fluorescence values of VSMC i in the Groups S0, S2 and S4 was significantly higher than that in the Group C ( P