Effects of GM1 on inducing adult rat bone marrow stromal cells to neural progenitor cells and their differentiation / 中华神经科杂志

ABSTRACT

Objective To investigate the effects of GMl on inducing adult rat MSCs to the neural progenitor cells and their descendants. Methods Purified MSCs were induced by basic fibroblast growth factor (bFGF) alone, GMl alone or by combination of bFGF and GMl. After 3 days of incubation, fibronectin and collagen I were detected by immunocytochemistry, and nestin by immunofluorescence. Neuron-specific enolase ( NSE) , glial fibrillary acidic protein ( GFAP) and galactose cerebroside ( GalC) were detected by immunocytochemistry after 7 days of incubation. Results After induction by bFGF alone or combination of bFGF and GM1, some MSCs exhibited the phenotypes of neural progenitor cells in 3 days, and then exhibited neurons and astrocytes in 7 days. In these two groups, cells positive for fibronectin and collagen I were decreased markedly after 3 days of induction. At the same time, cells positive for nestin were increased markedly. After 7 days of induction, NSE and GFAP-positive cells were increased significantly (22. 28% ?2. 97% and 26. 65%?3. 17% ). Furthermore, the combination of bFGF and GMl showed a maximal increase (30. 35%?3. 51% and 32. 22%?2. 68% ). But the GMl alone had shown no inductive effects. Conclusion Combination of bFGF and GMl might synergistically improve the neural induction of MSCs, showing a promising route for the application of MSCs.