Acceleraton of nerve regeneration following acellular allogeneic nerve grafting by bFGF / 中华显微外科杂志

ABSTRACT

Objective To evaluate the promoting effect by bFGF on nerve regeneration following acellular allogeneic nerve grafting,and the effects of various concentration of bFGF on axon growth. Methods The animals were divided into 1 000U/ml, 500U/ml, 250U/ml,100U/ml and normal saline groups. Immunohistochemistry staining of neurofilament-160 and S-100 was used to show the length of axonal growth and Schwann cell infiltration 10 days after the surgery. Results The average distance of regenerated nerve fibers in the high dose groups (1 000U/ml and 500U/ml) was longer than that in the normal saline control group;but there was no difference between the low dose groups (250U/ml and 100U/ml) and the control group. Conclusion Early use of bFGF when acellular allogeneic nerve grafted to bridge peripheral nerve defect may obviously promote axon growth speed.