Determination of the levels of Th1 and Th2 type cytokines in patients with unexplained habitual abortion / 中国免疫学杂志

ABSTRACT

Objective:To determine the levels of Th1 and Th2 type cytokines in patients with unexplained habitual abortion(UHA).Methods:A total of 54 patients with UHA were studied,including 35 cases of nonpregnant woman which were called UNP group and 19 cases of pregnant women with inevitable abortion(IEA) which were called UIEA group.15 cases of normal pregnant(NP) and 15 cases of normal nonpregnant(NNP) women were selected as control groups.Supernatants from trophoblast-activated peripheral blood mononuclear cells(PBMC) of nonpregnant women groups were tested by enzyme-linked immunosorbent assay(ELISA) for interferon gamma(IFN-?),interleukin-2(IL-2),IL-4,IL-10.Also,these cytokines were tested in the supernatants from PBMC and decidual mononuclear cells(DMC) of pregnant women groups.Results:①The levels of IL-2,IFN-? in trophoblast-activated PBMC supernatants of UNP group were higher and IL-4,IL-10 were lower than those of normal nonpregnant group( P