Expression of 5-LOX gene and its inhibitory effect in human pancreatic cancer cell / 中国现代普通外科进展
Chinese Journal of Current Advances in General Surgery
;
(4)1999.
Artigo
em Chinês
| WPRIM
| ID: wpr-539477
ABSTRACT
Objective:
To detect the expression of 5-LOX and observe its inhibitory effect in humane pancreatic cancer cell.Methods:
5-LOX mRNA and protein expression were analyzed by RT-PCR and immunocytochemical staining as well as images analyzing system,and the effect of MK886 was tested.Results:
MK886 effectively down-regulated the expression of 5-LOX mRNA.Immunocytochemical staining and images analysis confirmed that the expression 5-LOX protein in cell treated with MK886 was lower than untreated control cell.Conclusion:
Inhibitor of 5-LOX can effectively prevent the target mRNA into protein product,thus block or weaken the expression of 5-LOX.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Current Advances in General Surgery
Ano de publicação:
1999
Tipo de documento:
Artigo
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