Isolation of Phenol-degrading Bacteria and Analysis of ARDRA / 微生物学通报

ABSTRACT

A number of strains were isolated from the wastewater by direct plating.PCR with specific primer of phenol hydroxylase gene was used to identify the strains as phenol degrading bacteria.By this,a total of 87 phenol degrading strains were purified and divided into populations based on enterobacterial repetitive intergenic consensus sequence PCR(ERIC-PCR) genomic fingerprints.The results indicated that there were 15 different ERIC-PCR types present among the isolates.ARDRA with enzymes AluI revealed 4 operational taxonomic units(OTU) among 15 strains that exhibited different ERIC-PCR types.Moreover,the results indicate that there were 4 species at least.