Transportation function of calcium ion in sarcoplasmic reticulum of skeletal muscle in rat reduced training models after endurance training / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53)2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-540053
ABSTRACT
BACKGROUND:
Effect of exercise training on ultrastructure of skeletal muscle is a focus of quantified cytobiology in sports medicine.OBJECTIVE:
To explore the changes of transportation function of calcium ion in sarcoplasmic reticulum of muscle in the model of reduced training after endurance training. DESIGN, TIME ANDSETTING:
The randomized animal trial was performed at Xi'an Sports Institute from April to July 2006, and the biochemical test was performed at the Animal Experimental Center of Xi'an Jiaotong University in July 2006. MATERIALS Thirty-nine healthy 2-month-old male SD weighing (220?20) g were selected.METHODS:
Five rats were used in the preliminary experiment, and the others were randomly divided to normal control group (n=8), endurance training group (n=8) and reduced training group (n=18). The increasing training period of endurance training group and reduced training group lasted for 6 weeks, and then reduced training group was randomly divided to 3 groups according to reduced training cycle 2, 4 and 6 weeks group. The rats did increasing endurance loading exercise on a treadmill, while reduced intensity was used in reduced training group. MAIN OUTCOMEMEASURES:
Enzyme coupling method and fluorescence probe technology were used to detect sarcoplasmic reticulum Ca2+-ATPase (adenosine triphosphatase) and maximum Ca2+ uptake and release of gastrocnemius muscle.RESULTS:
The activity of Ca2+-ATPase in endurance training group was significantly increased compared with normal control group (P 0.05); reduced training 4 and 6 weeks groups were significantly reduced compared with endurance training group (P 0.05); while the maximum uptake and release of Ca2+ in sarcoplasmic reticulum of reduced training 4 and 6 weeks group was significantly reduced (P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Ensaio Clínico Controlado
Idioma:
Chinês
Revista:
Chinese Journal of Tissue Engineering Research
Ano de publicação:
2007
Tipo de documento:
Artigo
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