The effects of endothelin-1 and stem cell factor on melanocyte adhesion and migration in vitro / 西安交通大学学报(医学版)

ABSTRACT

Objective To study the effects of endothelin-1 ( ET-1) and stem cell factor (SCF) on melanocyte adhesion and migration in vitr o. Methods Human epidermal melanocytes that had been cultured and purified were treated with ET-1 and observed for adhesion to bovine serum f ibronectin-coated culture dishes. SCF and ET-1 treated cells were also examine d for migration into micropore filters coated with the same protein. Results Compared with SCF group, ET-1 treated melanocytes adhered to the dishes and moved into the filters more easily, especially when th e concentration was at 32 nmol?L -1. When the concentration of ET-1 was 128 mol?L -1 or more, melanocyte adhesion and migration were inhibited ( P