IL-1?,IL-6 levels and SOCS-3 gene expression in hypothalamus of BALB/c mice with brain asymmetry / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effects of brain asymmetry on levels of IL-1?,IL-6 and suppressor of cytokine signaling-3(SOCS-3) in hypothalamus,to get better understanding of relationship between brain asymmetry and neuro-immuno-endocrine network at the molecular level.Methods:By using paw preference test,right-pawed,left-pawed,and ambidextrous mice model was established.After decapitation of the mice,hypothalamus was separated rapidly.Some samples were homogenized and used for detection of levels of interleukin-1?(IL-1?),interleukin-6(IL-6) by Enzyme-Linked-Immunosorbent Assay(ELISA).The other samples were applied to detect SOCS-3 expression level by RT-PCR.Results:(1)The level of IL-6 in hypothalamusleft-pawed were higher than right-pawed mice,there was significant difference in statistics( P 0.05).(3)The expression level of SOCS-3the SOCS-3 expression of right-pawed showed higher than left-pawed mice in hypothalamus,revealing a significant difference( P