Inhibitory effect on human breast cancer SKBr-3 cells by RNA interference of survivin gene / 中国癌症杂志
China Oncology
;
(12)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-541121
ABSTRACT
Purpose:
To study the RNA interference-mediated inhibition of survivin gene on the proliferation of human breast cancer SKBr-3 cells.Methods:
SKBr-3 cells were transfected with a pSUPER-S1 vector plasmid that expressed survivin-targeted small interfering RNA, and the mRNA and protein levels of survivin gene were measured with RT-PCR and indirect immunofluorescence, respectively. The proliferation of transfected SKBr-3 cells was investigated through colony forming assay and MTT assay. The cell cycle phases were determined by flow cytometry(FCM).Results:
The mRNA and protein levels of survivin declined markedly in pSUPER-S1-transfected SKBr-3 cells. And the colony forming rate of those cells(38?16.70)% was significantly lower than that of the control cells(90.3?4.04)%.The growth of the pSUPER-S1-transfected cells was decelerated and the cell cycle was mainly blocked at G_(1) phase(74.03?8.91)%.Conclusions:
survivin gene silencing by RNA interference contributed to a distinctive inhibition of the proliferation of human breast cancer SKBr-3 cells in vitro.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Oncology
Ano de publicação:
2001
Tipo de documento:
Artigo
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