Experiment of ultrasound-mediated plasmid GFP transfer into H2K cell in vitro / 中华超声影像学杂志

ABSTRACT

Objective To explore the effect of ultrasound to gene transfer in skeletal muscle cells in mice. Methods All comparisons were performed between two intensities of ultrasound((0.5) W/cm~2, (1 W/cm~2)) with pulse wave (1 MHz, 20% duty cycle) using plasmid green fluorescent protein(GFP) on H2K myoblast cells. Exposure duration was 10 s, 20 s, 30 s, 40 s, 50 s, and 60 s. The trend of transfection efficiency by flow cytometer and cell viability by Trypan blue assays under different conditions was measured. Results GFP transfection rate in H2K cell treated by (0.5) W/cm~2 was significantly lower than that by 1 W/cm~2 as a whole. The optimum condition of GFP transfection for H2K cell was 1 W/cm~2 and (40-)50 s. Ultrasound did not increase the rate of cell-killing. Conclusions Ultrasound holds promise in gene delivery in skeletal muscle cells.