Identification of the type and the subtype of interferon with the application of protein biochips / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Artigo
em Chinês
| WPRIM
| ID: wpr-541328
ABSTRACT
Objective:
To establish a protein biochip flat to identify the subtype of interfon by adopting the 6 mAb which we have prepared.Methods:
Recombinant human interferon was onto the NHS modified gold biochips.then the protein biochips were incubated with the 6 mAb.Cy3 conjugated sheep anti-mouse IgG was used to detect the antigen-antibody.Arrays are imaged using a fluorescence scanner (GenePix4100A) at 532 nm for Cy3.This method was compared with the traditional onesELISA,Western blot.Results:
The results showed that this flat had speciality to identify the subtype of interfon.Conclusion:
The protein biochip plat can provide a practical method to identify the subtype of interfon.It also has some advantageshigh quantities;simple operation;low sample dosage and high repetition.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
1985
Tipo de documento:
Artigo
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