Preliminary study on human immune cells activated by CpG-ODN / 中国免疫学杂志

ABSTRACT

Objective:To study immunological stimulation of synthetic oligodeoxynucleotides(ODN) containing unmethylated CpG dinucleotides(CpG-ODN) on human immune cells.Methods:CpG-ODN was co-cultured with peripheral blood mononuclear cells(PBMC) .The IFN-? and IFN-? in the supernatant were measured by ELISA;the reverse transcription PCR was used to analyze the expression levels of TLR9 mR NA in PBMC;MTT method was used to detect NK-mediated lytic activity of K562 cells.Results:CpG-ODN induced high amounts of IFN-? as well as IFN-? production;the lysis of K562 mediated by CpG-ODN 2216 stimulating NK cells was increased;the CpG-ODN could up-regulate the expression of TLR9 mRNA in PBMC. Conclusion: CpG-ODN 2216 can activate human immune reaction by increasing the production of IFN-? and IFN-?,the expression of TLR9 and NK cell lytic activity.