Calcium mechanism of endoplasmic reticulum stress and the change of ultrastructures induced by rotenone in PC12 cells / 中华神经科杂志

ABSTRACT

Objective To investigate the calcium mechanism of endoplasmic reticulum stress and the changes of ultrastructure induced by rotenone. Methods Rotenone induced reactive oxygen species(ROS) production in PC12 cells were measured by using flow cytometry(FCM). Relative changes of intracellular calcium concentration were monitored by use of laser scanning confocal microscope. The ultrastructures were observed under transmitting electronic microscope. Results Treatment with different concentrations of rotenone ( 0.1 ?mol/L,1.0 ?mol/L,2.0 ?mol/L and 3.0 ?mol/L )increased the ROS generation in PC12 cells, and fluorescence intensity (FI) value was shown as 1.55?0.17, 2.16?0.10, 1.77?0.20 and 1.41?0.12, respectively. The same concentration of rotenone resulted in the elevation of intracellular calcium,the relative changes of fluorescence intensity about 0.6029?0.0685,1.0902?0.1127,0.7479?0.0820 and 0.5614?0.0870. There were significant differences as compared with those in control group (P