The effect of kidney-replenishing herb on the Indoleamine 2,3-dioxygenase of decidual macrophages / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-541505
ABSTRACT
Objective:
To study the effect of Kidney-replenishing herb on the indoleamine 2,3-dioxygenase(IDO) of decidual macrophages.Methods:
RT-PCR was applied to analyze the IDO mRNA transcription of decidue;Western blot was applied to analyze the expression of IDO protein on decidual macrophages;ELISA was applied to determinate the IL-10 and IFN-? concentration in culture supernates of decidual macrophages and lymphocytes; high-performance liquid chromatography was applied to determinate the kynurenine and tryptophan concentration in cell culture supernates, the ratio of kyn/try was used to determinate IDO activity.Results:
The decidual IDO mRNA transcription of normal pregnancy is higher than that of inevitable abortion; the inhibitor of IDO make the balance of Th cytokine depart from Th2; the serum made from herbal medicine improve the expression of IDO protein and IDO activity, and resume the balance of Th cytokine.Conclusion:
Appropriate IDO mRNA transcription and IDO activity of decidue is necessary for normal pregnancy;Kidney-replenishing herb improve IDO activity to appropriate value.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2001
Tipo de documento:
Artigo
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