Construction of enterotoxigenic Escherichia coli heat-stable enterotoxin fusion protein with glutathione S-transferase and detection of antibody against heat-stable enterotoxin / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2000.
Artigo
em Chinês
| WPRIM
| ID: wpr-541579
ABSTRACT
Objective:
To detection antibody against heat-stable enterotoxin by fusion protein.Methods:
Mutant heat-stable enterotoxin precursor gene was ligated in vector pGEX-4T-2 to inductively express as a fusion protein GST/proST_m with glutathione S-transferase(GST).To investigate the antigenic action,serum and fecal antibodies against heat-stable enterotoxin was detected with this fusion protein.Results:
The fusion protein was a about 32 kD protein.All the samples contain the antibody against ST.Conclusion:
Such strategy was a promising method to detect antibody against heat-stable enterotoxin.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2000
Tipo de documento:
Artigo
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