CEA DNA vaccine induces special cellular and humoral immune response to a carcinoembryonic antigen in mice / 中国癌症杂志

ABSTRACT

Purpose:To identify the expression of the DNA v accine in mice muscles and detect its ability to induce special cellular and hum oral immune response to carcinoembryonic antigens. Methods:Using cloning technique,the cDNA fragments of CEA gene and mouse co-stimulatory molecule IL-2 gene were constructed into the mammali an co-expression plasmid vector pIRES.Tongue muscle of mice were injected with our plasmid. The expression of CEA molecules in muscle was identified by immunoh istochemitry technique .The humoral responses were detected by ELISA technique, the special celluar immuse responses were detected by CTL and NK cytotoxity assa y. Results:We identified that our plasmids can express the CEA and IL-2 molecules in muscles of mice.CEA-antibody and IL-4 molecules appear in blood serum of mice,CTL activities and NK cytotoxity were much stronger in immun ized mice. Conclusions:The constructed mammalian co-expression plasmid pI RES-CEA , pIRES-IL-2,pIRES-CEA- IL-2 can express CEA and IL-2 molecules i n muscles and can generate cellular and humoral immunological responses to CEA p ositive carcinoma.