The effect of gtfB specific antisense oligodeoxyribonucleotides on S.mutans sucrose-dependent adherence to saliva coated hydroxyapatite in vitro / 实用口腔医学杂志

ABSTRACT

Objective:To evaluate the effect of gtfB specific antisense phosphorothioate-modified oligodeoxyribonucleotides(PS-ODNs) on S.mutans sucrose-dependent adherence.Methods:Antisense oligodeoxyribonucleotide targeted to gtfB sequence 709~726 bp(PS-ODN1) and 3 479~3 497 bp(PS-ODN2) were synthesized.Natural genetic transformation of S.mutans with PS-ODN1 and PS-ODN2 was respectively performed.Adhesion of S.mutans to saliva coated hydroxyapatite was examined by crystal violet staining and destain with absolute ethanol.The absorbance at 620 nm was measured by plate reader(the absorbance value derived from the wells without sucrose was used as background and was subtracted).Results:The adhesion ability of the strains treated with antisense PS-ODN was significantly lower than that of the control(P