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Detection of Potential Microcystin-Producing Cyanobacteria in Waters With a mcyD Molecular Biomarker / 环境与健康杂志
Article em Zh | WPRIM | ID: wpr-542775
Biblioteca responsável: WPRO
ABSTRACT
Objective To develop a new method to screen the potential microcystin-producing cyanobacteria in natural waters. Methods Cyanobacteria were cultured and waters samples were analyzed by polymerase chain reaction with special primers for gene of the operon mcyD which encodes a microcystin synthetase combining conserved gene 16S rRNA of alga, subsequently, whose microcystins were detected by ELISA. Results There was a unique amplified product of approximately 870 bp in microcystin-producing blue-green alga and water samples, but no target band in non-toxic strains and samples. Conclusion It is feasible to use the molecular biological method used mcyD as the microcystin molecular biomarker to screen microcystin-producing cyanobacteria in natural water.
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Texto completo: 1 Índice: WPRIM Tipo de estudo: Diagnostic_studies Idioma: Zh Revista: Journal of Environment and Health Ano de publicação: 1992 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Tipo de estudo: Diagnostic_studies Idioma: Zh Revista: Journal of Environment and Health Ano de publicação: 1992 Tipo de documento: Article