Regulation of osteoprotegerin expression by CSF-1 in human dental follicle cells / 实用口腔医学杂志

ABSTRACT

Objective:To study the effects of colony-stimulating factor-1 (CSF-1) on the expression of osteoprotegerin (OPG ) in human dental follicle cells.Methods:Human dental follicle cells culture was established and cell crawling slides were stained by OPG antibody. The third to sixth passage cells were incubated with 25 ng/ml CSF-1 for 0.5,1,3 and 6 h respectively,then the conditioned medium and cells were collected for ELISA and RT-PCR assay respectively.Results:OPG was expressed in the cytoplasm of human dental follicle cells.GSF-1 at 25 ng/ml and 1 h-exposure down-regulated OPG expression in the cells(P