MRI in Evaluation of Transplantation of Autologous Marrow Stromal Cells Transfected Ex Vivo by VEGF Gene in a Porcine Chronic Myocardial Ischemia / 实用放射学杂志

ABSTRACT

Objective To investigate the evaluation of multimodality MR imaging on the transplantation of autologous marrow stromal cells transfected ex vivo by vascular endothelial growth factor(VEGF)gene in a porcine chronic ischemic heart disease model. Methods Pigs of chronic ischemic heart disease model were randomly divided into two groups treatment with marrow stromal cells transfected ex vivo by VEGF(Group I,n=9) and treatment with adenovirus served as control(Group Ⅱ,n=7).Four weeks after therapy,ejection fraction,ischemic segment and infracted segment were detected by multimodality MR imaging.The percentage of infarction area,CM-DiI labeled cells on fluorescence microscopy,vessel density were detected on specimen. Results In Group Ⅰ,in comparison with Group Ⅱ,the ejection fraction was significantly improved,the ischemic and infarction segments decreased(P