Effect of carboxymethyl-chitosan on proliferation and collagen synthesis in keloid fibroblasts in vitro / 中华医学美学美容杂志
Chinese Journal of Medical Aesthetics and Cosmetology
;
(6)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-543265
ABSTRACT
Objective To investigate the effects of carboxymethyl-chitosan (CM-CH) on keloid fibroblasts (KFB) proliferation and collagen synthesis. Methods Keloid fibroblasts were isolated from fresh keloid tissue and cultured. The effect of CM-CH on proliferation was examined by MTT assay. The synthesis of collagen was evaluated by hydroxy proline (HP) colorimetric analysis. Results The fibroblasts were treated with CM-CH at concentration of 10 ?g/ml, 50 ?g/ml, and 100 ?g/ml 48h and 72h after treatment, and all of the three concentrations showed inhibitory effects significantly (P0.05) at 200 ?g/ml concentration after 24h, 48h, and 72h. CM-CH at concentration of 10 ?g/ml, 50 ?g/ml, and 100 ?g/ml after treatment for 48h on KFB could markedly decrease the synthesis of collagen (P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Medical Aesthetics and Cosmetology
Ano de publicação:
2001
Tipo de documento:
Artigo
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