Effect of retinoic acid on the secretion of C3 and factor B in lung carcinoma cell line A549 induced by TNF-? and IL-6 / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Artigo
em Chinês
| WPRIM
| ID: wpr-543313
ABSTRACT
Objective:
To study the effect of retinoic acid(RA) which is used in malignant tumor therapy on the secretion of C3 and factor B in human lung cancer cell line A549 induced by TNF-? and IL-6.Methods:
C3 and factor B in the culture medium were measured by ELISA and Western blot,the mRNA expression of C3 and factor B was analyzed by RT-PCR.Results:
When using TNF-?(10 ?g/L),IL-6(50 ?g/L) and RA(1 ?mol/L),the results of ELISA,Western blot and RT-PCR showed that TNF-? and IL-6 promoted A549 cells excreting C3 and factor B and their mRNA expression;RA alone did not affect C3 and factor B;in TNF-? group and TNF-?+RA group,the amounts of C3 were 91.40?12.59 and 133.59?11.25(ng/10~6 cells) respectively(P0.05),and the amounts of factor B were 13.07?2.50 and 32.89?4.22(ng/10~6 cells) respectively(P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
1985
Tipo de documento:
Artigo
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