Your browser doesn't support javascript.
loading
The Effects of Transforming Growth Factor beta1 on Apoptosis in Rat Hepatocellular Carcinoma
Korean Journal of Pathology ; : 71-79, 1999.
Artigo em Coreano | WPRIM | ID: wpr-54344
ABSTRACT
Based upon the concept that carcinogenesis is associated with apoptosis, specific therapies designed to enhance the susceptibility of cancer cells to undergo apoptosis could be developed. Thus, in this paper, it was designed to investigate whether, using rat animal model with chemical-induced hepatocellular carcinoma, TGF-1 in vivo could induce apoptosis in cancer. The chemical hepatocarcinogenic procedure of Solt-Farber method was used on Sprague-Dawley rats. Experimental groups were divided into group A treated with the standard Solt-Farber regimen of diethylnitrosamine (DEN) and 2-Acetaminofluorene (AAF), group B TGF-, group C TGF-1, and group D adriamycin after hepatocellular carcinoma developed. For detection of apoptotic cells, apoptotic indices were examined by the in situ end DNA labelling method. The expression of proliferating cell nuclear antigen was examined by immunohistochemical staining. Apoptosis of rat hepatocellular carcinoma cells increased significantly to 4.92+/-2.32/HPF in the group C compared with the control group (A) (2.54+/-1.13/HPF; P<0.05). Two distinctly different populations of proliferating hepatocellular carcinoma cells were identified. The cells at G1/S boundary (weak granular staining) increased to 15.75+/-6.19/HPF and 6.45+/-2.93/HPF in the groups C and D, respectively, but decreased to 2.42+/-2.06/HPF in the group B compared with the control group (A) (6.38+/-2.18/HPF; p<0.05). The cells at S phase (strong granular staining) increased to 3.37+/-2.69/HPF in the group B but decreased to 0.32+/-0.47/HPF in the group D (p<0.05). In conclusion, these results indicate that the TGF-1 may be used as an effective anticancer agent.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: DNA / Fatores de Crescimento Transformadores / Doxorrubicina / Fase S / Fator de Crescimento Transformador alfa / Ratos Sprague-Dawley / Apoptose / Carcinoma Hepatocelular / Antígeno Nuclear de Célula em Proliferação / Modelos Animais Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Coreano Revista: Korean Journal of Pathology Ano de publicação: 1999 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: DNA / Fatores de Crescimento Transformadores / Doxorrubicina / Fase S / Fator de Crescimento Transformador alfa / Ratos Sprague-Dawley / Apoptose / Carcinoma Hepatocelular / Antígeno Nuclear de Célula em Proliferação / Modelos Animais Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Coreano Revista: Korean Journal of Pathology Ano de publicação: 1999 Tipo de documento: Artigo