DNA Damage Induced by Amatoxins and Antagonism of Eicosapentaenoic Acid, Docosahexaenoic Acid in Rat Histiocytes / 环境与健康杂志

ABSTRACT

Objective To study the adverse effect of amatoxins on DNA in the histiocytes of rats and the antagonism of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA). Methods SD rats were randomly divided into three groups, the number of males and females was the same. The rats in the experimental groups were respectively treated with amatoxins(0.1 mg/kg bw, i.p.) and amatoxins added EPA, DHA (both were 10 mg/kg bw, i.p.) for 10 days. Single cell gel-electrophoresis was employed to test the damage of DNA in the histiocytes. Results In the group treated with amatoxins, DNA damage in the peripheral lymphocyte, hepatocytes, lung cells ,brain cells was more seriously compared with the control, but no significant DNA damage was seen in the group treated with amatoxins added EPA, DHA. Conclusion Amatoxins may induce DNA damage and eicosapentaenoic acid, docosahexaenoic acid have an antagonism to the adverse effect of amatoxins.