Effects of minocycline at subantimicrobial dosage in the treatment of experimental periodontitis in rats / 实用口腔医学杂志

ABSTRACT

Objective: To evaluate the effectiveness of minocycline at subantimicrobial dosage in the treatment of experimentally induced periodontitis in rats.Methods: Periodontitis was induced by silk ligature technique on 4 second molars in each of 16 adult male Sprague-Dawley rats and the animals were fed with 100 g/L sucrose drink. The rats were divided into 2 groups (1)periodontitis group without treatment;(2)treatment group, the rats with periodontitis were treated by systemic administration of minocycline at 5 mg/(kg?d).Another 8 normal rats were used as the controls. Assessment was carried out at day 28 and 56 using a number of different visual, histological and ultrastructure approaches.Visual assessment included tooth mobility(TM), gingival index(GI), and alveolar bone loss. Histological examination included monocyte effusion,resorption lacunae with osteoclasts and percentage of the periodontal collagen.The collected data were statistically analyzed using variance test.Results: Systemic administration of minocycline at subantimicrobial dosage can significantly reduce GI, TM, resorption lacunae with osteoclasts and alveolar bone loss either at day 28 or at day 56; significantly inhibited monocyte effusion and the collagen degradation in the periodontium at day 56 in rats with periodontitis. Conclusion: Minocycline at subantimicrobial dosage may decrease alveolar bone loss and osteoclasts formation in periodontium with periodontitis.