Influence of continuous pressure on the function of rabbit bone marrow stromal cells proliferation / 中国矫形外科杂志

ABSTRACT

[Objective]To investigate the influence of pressure on the proliferation of rabbit bone marrow stromal cells(MSCs) and explain the relationship of the hip prosthesis submerge and loosening with continuous pressure after revision of total hip replacement.[Method]A unit of MSCs pressure model was set up to load different pressures on MSCs cultured in vitro.[Result]MSCs showed more proliferation capacity under discontinuous pressure.MTT assay was used to determine the cell proliferation of primarily cultured MSCs under discontinuous pressure.The MSCs OD value of pressured groups was much smaller than the control group at different times.Various magnitudes and durations of the discontinuous pressure could significantly suppress MSCs proliferation with the magnitude-dependent.[Conclusion]At the early period after revision of total hip replacement,patients should lie and exercise on the bed until 6 weeks.Otherwise,the weight-bearing pressure may restrain MSCs proliferation which is harmful to union and may cause prosthesis submerge and loose.