A control study of S.mutans genotype in the oral cavity of mothers-children pairs / 实用口腔医学杂志

ABSTRACT

Objective:To study the relationship between genetic diversity within S.mutans and dental caries.Methods:Isolates of S.mutans were obtained from 10 caries active children and their mothers,and were originally isolated on mitis-salivarious-bacitracin agar.A total of 800 isolates were biochemically confirmed as S.mutans.Chromosomal DNA was extracted from those isolates with an extraction kit.By AP-PCR,the genetic diversities of S.mutans were examined respectively in 10 caries active children,10 caries-free children,and their mothers.DNA fingerprints were obtained by electrophoresis on 15 g/L agarose gel and analyzed for genotypics similarities.Results:The number of distinct genotypes of S.mutans harbored in caries active children were,on average,greater than that presented in children without caries(P