The effect of Bifidobacterium BB12 on S.enteritidis-induced IL-8 secretion by Caco-2 cells / 中国免疫学杂志

ABSTRACT

Objective:To investigate the in vitro effect of Bifidobacterium BB12 on IL-8 secretion by Caco-2 cells induced with S.enteritidis.Methods:The coculture systems of Caco-2 cells with S.enteritidis alone or combined with the strain of Bifidobacterium BB12 were established. The expression of IL-8 mRNA and NF-?B p65 in the cells were examined by reverse transcription PCR, real-time PCR and EMSA, and IL-8 in the supernatant was measured by ELISA.Results:In control group, there was little IL-8 mRNA expression in Caco-2 cells, NF-?B p65 was seldom in Caco-2 cells’ nuclei, and the concentration of IL-8 in the supernatant was as few as 126 ng/L. After stimulated by the S.enteritidis, the expression of IL-8 mRNA and activated NF-?B p65 was found in Caco-2 cells (P