Study on recombinant chicken ? interferon production in yeast Pichia pastoris and detection for its antiviral activity / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2000.
Artigo
em Chinês
| WPRIM
| ID: wpr-545430
ABSTRACT
Objective:
To obtain eukaryotic expressing protein of chicken interferon ? (ChIFN-?) and research its anti-virus activity.Methods:
Chicken interferon ? mature protein gene was cloned and amplified by reverse transcripition-polymerase chain reaction(RT-PCR) from the total mRNA in the lymphocyte of chicken blood stimulated with ConA for 4~10 hours.The gene was inserted into the expression vector pPICZa-A,which had been cleaved by EcoR I and Xba I.The recombinant vector was linearized by Sac I and transferred into yeast Pichia pastoris,strain X33,anti-virus activity of the recombinant cytokine was detected by the classical experiment cell pathological effect inhibition assay.Results:
The result showed that the preparation of recombinant interferon had higher anti-virus activity(10?48 U/ml).Conclusion:
The recombinant chicken interferon ? with anti-virus bioactivity has been obtained.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2000
Tipo de documento:
Artigo
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