Role of NF-?B p50 protein in pathologic responses of experimental acute pancreatitis in mice / 中国现代普通外科进展
Chinese Journal of Current Advances in General Surgery
;
(4)2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-545476
ABSTRACT
Objective:
To observe the roles of p50 protein in pathologic responses of cerulein(CR)pancreatitis in mice deficient in p50 protein of NF-?B.Methods:
Pancreatitis was induced by i.p.injection of 50?g/kg CR.The express and compensation of p65,p50 and cRel proteins of NF-?B,NF-?B activity were measured using electromobility shift assay;parameters of pancreatitis,eg,amylase,lipase and trypsin were evaluated.The apoptosis,necrosis and caspase-3 activity were observed.Results:
p50 knockout(KO)mice had no p50 protein expression and increased p65 and cRel proteins expression.p50 KO mice's NF-?B activity decreased and apoptosis increased in pancreatitis compared with wild type(WT)mice.There was no caspase-3 activition in both mice before and after pancreatitis.All KO mice had an increased necrosis and amylase,lipase,trypsin activity compared with WT mice in pancreatitis.Conclusions:
The results indicated that NF-?B p50 genetic deletion might attenuate cerulean induced pancreatitis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Current Advances in General Surgery
Ano de publicação:
2004
Tipo de documento:
Artigo
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