Preventing acute rejection after liver transplantation via blockading B7/CD28 costimulatory pathway in rats / 中国现代普通外科进展

ABSTRACT

Objective:To observe the effect of CTLA-4Ig on preventing acute rejection and the role of costimulatory molecule B7-1 and B7-2 via the model of orthotopic liver transplantation(ROLT)in rats.Methods:Build the model of ROLT(DA-Lewis).All rats were divided into 2 groups randomly.Group A was control group.Group B was injected with CTLA4-Ig into abdominal cavity after 48 hours of operation.Animals were sacrificed on the day 3,5,7,10 after ROLT to detect the changes of serum biochemistry(ALT,TBIL and DBIL).Liver grafts were collected for measuring the expression of B7-1 and B7-2 mRNA by RT-PCR.Results:1)Compared with the control group,the expression of B7-1 and B7-2 mRNA increased significantly(P