Experimental study of treating osteochondral defects with osteochondral composites constructed in vivo / 中国矫形外科杂志

ABSTRACT

[Objective]To evaluate the effect of treating the osteochondral defects with implanted cell-scaffold composites,cultured MSCs as seed cells and PLGA as scaffolds,and to acquire desirable seed cells and scaffold materials.[Method]BMSCs were induced to differenciatiated into chondrocytes,co-cultured with PLGA scaffold respectively in vitro,then implanted into osteochondral defects on canine models by using techniques of mosaicplasty,induced BMSC-PLGA scaffold composites in the top of the defect and BMSC-PLGA scaffold composites in the bottom of the defect,osteochondral composites were constructed in vivo,and repair was observed with naked eyes and histology.[Result]At 16 weeks after transplantation the defects of expeirmental group were covered with semi-transparent smooth white tissue and the margins between the repair tissue and the surrounding cartilage were not recognized.Histologically,most of the repair tissue was consisted with chondrocytes,maintained their thickness to the full depth of the original defects.The subchondral bone was well remodeled.The tidemark was observed.The defects of positive control group were covered with repair tissues,and partial were conformed with original cartilage.The repair tissue was partly filled with chondrocytes.However,the defects of negative control group were repaired with soft fibrous tissues without luster,and an obvious boundary between reparative and original cartilage was seen.[Conclusion]MSCs-PLGA scaffold composites,constructed into osteochondral composites by suppressing closely in vivo,are the ideal materials for repairing cartilage defects.